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citG [2018-02-10T14:03:31.000Z]
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citG [2018-02-10T14:03:31.000Z]

fumarase
Locus
BSU33040
Isoelectric point
5.47
Molecular weight
50.36 kDa
Protein length
462 aa Sequence Blast
Gene length
1386 bp Sequence Blast
Function
TCA cycle
Product
fumarate hydratase
Essential
no
E.C.
4.2.1.2
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Carbon core metabolism → TCA cycle

    • Gene

    Coordinates
    3,389,024 → 3,390,412

    The protein

    Catalyzed reaction/ biological activity

  • (S)-malate = fumarate + H2O (according to Swiss-Prot)

    • Protein family

  • Fumarase subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • AnsB

    • Structure

  • 1FUO (from E. coli, 64% identity) PubMed

    • Localization

  • forms discrete foci in the cytoplasm PubMed
  • colocalizes with DNA upon DNA damage (MMS treatment) PubMed

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    citG
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigH: sigma factor, PubMed, in SigH regulon

    • Regulation

  • constitutive
    • view in new tab

    Genes
    yvzF-citG
    Description
    PubMed
    view in new tab

    Additional information

  • the protein amounts increase upon DNA damage (MMS treatment) PubMed

    • Biological materials

    Mutant

  • GP718 (spec), available in Jörg Stülke's lab PubMed
  • GP2340 (citG::kan), Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
  • GP2341 (citG::lox72), Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
  • BKE33040 (ΔcitG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTATGTATCCCTCCATA, downstream forward: _UP4_TAATAGGAAGAACGGCTGCT
  • BKK33040 (ΔcitG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTATGTATCCCTCCATA, downstream forward: _UP4_TAATAGGAAGAACGGCTGCT

    • Expression vector

  • pGP1122 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab) PubMed

    • LacZ fusion

  • pGP387 (in pAC7), available in Jörg Stülke's lab

    • GFP fusion

  • GP1433 (spc, based on pGP1870), available in the Stülke lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • FLAG-tag construct

  • GP1132 (spc, based on pGP1331), available in Jörg Stülke's lab

    • Antibody

  • **

    • References

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